Sexing Greater Flamingo Chicks from Feather Bulb DNA

Özge Balkiz; Stéphanie Dano; Christophe Barbraud; Serpil Tekin; Uygar Özesmi; Munis Dündar; Arnaud Béchet

doi:10.1675/1524-4695(2007)030[0450:SGFCFF]2.0.CO;2

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Özge Balkiz,^1,2,3 Stéphanie Dano,⁴ Christophe Barbraud,⁴ Serpil Tekin,⁵ Uygar Özesmi,⁶ Munis Dündar,⁵ Arnaud Béchet¹

¹Station Biologique de la Tour du Valat, Le Sambuc, 13200 Arles, France
²CEFE, Equipe Biométrie et Biologie des Populations, 1919 Route de Mende, 34293 Montpellier, France
³Doga Dernegi, Kennedy Cad., No: 50/19, Boylu apt. (06660), Kavaklıdere, Ankara, Turkey. Corresponding author; ozge.balkiz@dogadernegi.org
⁴CEBC-CNRS UPR 1934, Villiers-en-Bois, F-79360 Beauvoir Sur Niort, France
⁵Erciyes University, Medicine Faculty, Department of Medical Genetics, 38039 Kayseri, Turkey
⁶Erciyes University, Engineering Faculty, Environmental Engineering Department, 38039 Kayseri, Turkey

Abstract

Adult Greater Flamingos (Phoenicopterus roseus) are sexually dimorphic, with males being on average larger and heavier than females. However, there is no practical way to sex the chicks by their morphology. Here we describe a method relying on quick and easy DNA extraction from feathers. A PCR test employing primers to amplify introns whose lengths usually differ between the CHD-W and the CHD-Z genes allow sex discrimination. This method is thus a fast, accurate and inexpensive protocol to sex flamingo chicks from feathers bulbs sampled in the field.

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Özge Balkiz, Stéphanie Dano, Christophe Barbraud, Serpil Tekin, Uygar Özesmi, Munis Dündar, and Arnaud Béchet "Sexing Greater Flamingo Chicks from Feather Bulb DNA," Waterbirds 30(3), 450-453, (1 September 2007). https://doi.org/10.1675/1524-4695(2007)030[0450:SGFCFF]2.0.CO;2

Received: 19 July 2006; Accepted: 1 December 2006; Published: 1 September 2007

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CHD

CHD-W

CHD-Z

feather bulb

PCR based protocol

Phoenicopterus roseus

polymerase chain reaction

sex determination

sexing

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